Chromatography

While LC-NMR and LC-MS are useful analytical methods, for the structural analysis of compounds, the conditions most often used for HPLC are not optimum for NMR or MS. For that reason, analysis of samples in a complex matrix require cumbersome procedures involving initial separation-purification using HPLC, followed by desalting and other processes before NMR/MS analysis can be performed.

Co-Sense LC-NMR / LC-MS is an automated sample pretreatment system designed to facilitate the processing of complex samples and augment the efficiency of NMR and MS measurements. HPLC column-switching technology is used to separate, purify and concentrate target compounds, and perform desalting and solvent replacement. This eliminates the bother and expense of performing such operations manually. Further, the separation of target compounds using HPLC is not limited by NMR and MS restrictions, allowing HPLC parameter selection as desired.

System is designed for LC-NMR and LC-MS sample preparation. After a semi-preparative or analytical separation the peaks can be collected and pre-concentrated on a trap column to reach the necessary concentration for an NMR analysis. The expansive D²-solvents will be used only in the last step, when the samples are re-eluted after trapping. Full automation allow online or offline measurements and can reduce the time for manual preparation.

For LCMS use the separation can be done with typical high concentrated buffers, the solvents will be exchanged direct before the LCMS inlet. Especially if LCMS is not the standard method and is asked to confirm the results of other techniques this could be an alternative, because validated methods are not need to change.